#Protein ladder western blot software

Although Image Studio Software is no longer in development, we continue to sell and support it. Using protein ladders specifically designed for fluorescent western blotting can help balance the fluorescent signals. Image Studio™ Softwareįor instructions on entering marker molecular weights and sizing bands in Image Studio Software, visit /bio/support. The dyes used to make prestained molecular weight markers often have some fluorescent properties, however their signals can potentially overwhelm the fluorescence of the target proteins.

For more information, see the instructions for the Experiment workflow you are using at /WesternWorkflowOverview. Molecular weights for WesternSure Chemiluminescent Pre-Stained Protein ladders can be sized in Empiria Studio Software. The WesternSure Pre-stained Chemiluminescent Protein Ladder is not a standard and molecular weights may vary, depending on the gel type.

#Protein ladder western blot verification

These are extensively used in gel electrophoresis and western blot assays to monitor protein movement and quantify bands. Visualization of protein migration during SDS-polyacrylamide gel electrophoresis Verification of protein transfer onto membranes after Western blotting. Some of the most common and widely used include prestained, unstained, western blot, and fluorescent protein ladders. Stripping buffer alters the chemiluminescent functionality of the ladder, resulting in weak or no signal. Protein ladders are available in different types. The ladder is not recommended for use when stripping and reprobing Western blots. Mix the product thoroughly before use to dissolve any solids that may have precipitated during storage. Bring the product to room temperature while protecting from light. WesternSure Pre-stained Chemiluminescent Protein Ladders are provided ready to use in loading buffer with no dilution, heating, or reconstitution required.

To see a complete portfolio of LI-COR molecular weight markers, visit /proteinmarkers. The ladder is optimized for use with Bis-Tris and Tris-Glycine systems. In blotting applications, the ladder can be used to monitor protein transfer and as a reference to estimate the molecular weight of proteins of interest. In gels, the ladder can be used to visualize progress of the protein separation during electrophoresis and to estimate the molecular weight of unknown proteins based on their relative mobility. The ladder offers both pre-stained and chemiluminescent functionalities and is suitable for use with film and a variety of chemiluminescent substrates.

The WesternSure Pre-stained Chemiluminescent Protein Ladder provides a ladder of convenient and consistent protein sizes (8 - 260 kDa) for use with polyacrylamide gels and on Western membranes where chemiluminescent detection is used. Depending on the primary antibody, is sometimes possible to detect also the protein ladder, this does not means that your western blot is wrong or that the bands that you see are unspecific.